Gene Editing Platform — Multi-Strategy Precision Genome Engineering

 The Gene Editing Platform integrates three complementary editing systems — Cas9, Base Editor (A/C-type), and SpRY variant — to achieve broad target coverage and high editing efficiency.

For target regions adjacent to PAM sites and suitable for short-fragment deletions, the platform employs the highly efficient Cas9 nuclease system. For loci requiring precise single-base substitutions, customized base editing tools enable accurate A→G or C→T conversions. For target sites lacking canonical PAM sequences, the SpRY system enables “PAM-free” precision editing, expanding the editable genome space.

To streamline downstream identification, a fluorescent (RFP) marker module is integrated into the vector design, allowing early screening of positive and vector-free plants. This strategy significantly improves the efficiency of isolating non-transgenic edited lines, ensuring both precision and practicality in crop genome engineering.